SCIENCE-JOBS-DE Stellenausschreibung (vacancies)
----------------------------------------------------------------------

Dieses Angebot wurde eingegeben bei http://www.drarbeit.de,
der Internet-Stellenboerse und Kooperationspartner von
SCIENCE-JOBS-DE für Diplom- und Promotionsarbeiten
----------------------------------------------------------------------

Fachgebiete: Biologie Informatik Mathematik Physik
Thema: Doktorarbeit: High Resolution Single Particle Cryo EM of Hsp100 Assemblies
Ansprechpartner: Dr. rer. nat. Petra Wendler
Antwort an: wendler@lmb.uni-muenchen.de
Institution: Gene Center LMU Munich
Ort: 81377 Muenchen, Feodor-Lynen-Str. 25

----------------------------------------------------------------------

Our lab is interested in the conformational dynamics of multi-subunit ATPase complexes of the AAA+ (ATPases associated with various cellular activities) superfamily. We aim to obtain a structural view of these molecular machines by combining single particle cryo electron (EM) microscopy with biochemical and biophysical analysis.
The aim of the project is to solve the structures of ClpV and ClpC (Hsp100) oligomers at the highest possible resolution using cryo EM. By recording structural snapshots of their movements we want to understand the working mechanism of the oligomer. Focus in this project will be to optimize current data collection and image processing steps to achieve high resolution cryo EM structures.

Methoden:
single particle cryo EM, image processing, 3D reconstruction, atomic structure fitting,

Anfangsdatum: 1. Mai 2010

geschätzte Dauer: 3.5 Jahre

Bezahlung: BATII/2

Veröffentlichungen:
Wendler P, Saibil H (2010). Cryo electron microscopy structures of Hsp100 proteins- crowbars in or out? Biochem. Cell Biol. 88:89-96.

Wendler P, Shorter J, Snead D, Plisson C, Clare DK, Lindquist S, Saibil H (2009). Motor mechanism for protein threading through Hsp104. Molecular Cell. 34. 81-92.

Wendler P, Shorter J, Plisson C, Cashikar A, Lindquist S, Saibil H (2007). Atypical AAA+ subunit packing creates an expanded cavity for disaggregation by the protein-remodeling factor Hsp104. Cell 131(7):1366-77.

Puri T, Wendler* P, Sigala B, Saibil H, Tsaneva IR (2007). Dodecameric structure and ATPase activity of the human TIP48/TIP49 complex. J Mol Biol. 366(1):179-92.

Homepage: http://www.lmb.uni-muenchen.de/wendler/_welcome.html

We are looking for a highly motivated individual with experience in bioinformatics and/or structural biology. You should have a University degree in computer science/bioinformatics or mathematics/statistics, and have an interest in biology and biophysical techniques.

The Gene Center of the University of Munich is a multi-disciplinary center for world-class research and teaching. We offer a stimulating environment, including an open minded, ambitious young team and state-of-the-art cryo EM facilities. Please send your application in English or German by email. The application should comprise a cover letter with a brief statement of research experience and interests, a CV as well as undergraduate and graduate certificate.

----------------------------------------------------------------------
Mehr Informationen zu SCIENCE-JOBS-DE finden Sie im WWW unter der
Adresse http://jobs.uni-hd.de, insbesondere zu den Themen An/Abmelden,
Informationen fuer Anbieter und "Netiquette" - more information on
SCIENCE-JOBS-DE is available on the Web at http://jobs.uni-hd.de,
especially regarding subscription and unsubscription, information for
offerings and "netiquette".

googlefacebookdiggdel.icio.usredditfurl